CAT#:
13-045
Intact Genomics
BL21(DE3) pLysS Competent Cells
Chemically Competent Cells, 12 x 50µl/Unit
Chemically Competent Cells, 12 x 200µl/Unit
Electrocompetent Cells, 6 x 100µl/Unit
Electrocompetent Cells, 12 x 100µl/Unit
4 product variations: View Options
Product Overview
- Suitable for transformation and routine protein expression
- Carry the chloramphenicol resistance gene
- Has T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
- Suitable for expression of toxic genes
Product Overview
- Suitable for transformation and routine protein expression
- Carry the chloramphenicol resistance gene
- Has T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
- Suitable for expression of toxic genes
Product Overview
- Suitable for transformation and routine protein expression
- Has T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
- Suitable for expression of toxic genes
- Includes: ig® BL21(DE3)pLysS electroCompetent cells, pUC19 control DNA, and recovery medium
Product Overview
- Suitable for transformation and routine protein expression
- Has T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
- Suitable for expression of toxic genes
- Includes: ig® BL21(DE3)pLysS electroCompetent cells, pUC19 control DNA, and recovery medium
Technical Specifications
| Competent Cell Type | Chemically competent |
| Derivative Of | BL21(DE3)pLysS |
| Species | E. coli |
| Format | Tubes |
| Transformation Efficiency | ?3 x 10^7 cfu/µg pUC19 DNA |
| Blue/white Screening | Yes |
| Shipping Condition | Dry ice |
Technical Specifications
| Competent Cell Type | Chemically competent |
| Derivative Of | BL21(DE3)pLysS |
| Species | E. coli |
| Format | Tubes |
| Transformation Efficiency | ?3 x 10^7 cfu/µg pUC19 DNA |
| Blue/white Screening | Yes |
| Shipping Condition | Dry ice |
Technical Specifications
| Competent Cell Type | Electrocompetent |
| Derivative Of | BL21(DE3)pLysS |
| Species | E. coli |
| Format | Tubes |
| Transformation Efficiency | ?1 x 10^10 cfu/µg pUC19 DNA |
| Blue/white Screening | Yes |
| Shipping Condition | Dry ice |
Technical Specifications
| Competent Cell Type | Electrocompetent |
| Derivative Of | BL21(DE3)pLysS |
| Species | E. coli |
| Format | Tubes |
| Transformation Efficiency | ?1 x 10^10 cfu/µg pUC19 DNA |
| Blue/white Screening | Yes |
| Shipping Condition | Dry ice |
Technical Documents
Technical Documents
Technical Documents
Detailed Description
Intact Genomics (ig®) BL21(DE3)pLysS competent cells are suitable for transformation and routine protein expression. The pLysS plasmid produces T7 lysozyme that reduces base-level expression of the gene of interest, subsequently allowing for tighter control of expression and is, therefore, suitable for the expression of toxic genes. BL21(DE3)pLysS chemically competent cells also carry the chloramphenicol resistance gene.
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high efficiency transformation protocol. Transformation efficiency should be greater than 3×10^7 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
4 Product Options
Catalog Number:
13-014
List Price:
$249.65
Your Price:
$213.99
Specifications:
| Cell Competence | Chemically Competent |
| Volume | 12 x 50µl |
| Description | Chemically Competent Cells |
| Qty/Unit | 12 x 50µl/Unit |
Catalog Number:
13-015
List Price:
$493.44
Your Price:
$422.94
Specifications:
| Cell Competence | Chemically Competent |
| Volume | 12 x 200µl |
| Description | Chemically Competent Cells |
| Qty/Unit | 12 x 200µl/Unit |
Catalog Number:
13-044
List Price:
$518.57
Your Price:
$444.49
Specifications:
| Cell Competence | Electrocompetent |
| Volume | 6 x 100µl |
| Description | Electrocompetent Cells |
| Qty/Unit | 6 x 100µl/Unit |
Catalog Number:
13-045
List Price:
$906.14
Your Price:
$776.69
Specifications:
| Cell Competence | Electrocompetent |
| Volume | 12 x 100µl |
| Description | Electrocompetent Cells |
| Qty/Unit | 12 x 100µl/Unit |
| Catalog # | Description | Qty/Unit | Cell Competence | Volume | Price (USD) | Quantity | Add to Order | |
|---|---|---|---|---|---|---|---|---|
| 13-014 | Chemically Competent Cells | 12 x 50µl/Unit | Chemically Competent | 12 x 50µl | ||||
| 13-015 | Chemically Competent Cells | 12 x 200µl/Unit | Chemically Competent | 12 x 200µl | ||||
| 13-044 | Electrocompetent Cells | 6 x 100µl/Unit | Electrocompetent | 6 x 100µl | ||||
| 13-045 | Electrocompetent Cells | 12 x 100µl/Unit | Electrocompetent | 12 x 100µl |
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