CAT#:
11-724B
Zymo Research
ZymoBIOMICS™ 96 MagBead DNA Kits
No Lysis Matrix, 2 x 96 Preps/Unit
Zymo Research, 4 x 96 Preps/Unit
Zymo Research, 96 Preps/Unit
Lysis Rack, 2 x 96 Preps/Unit
Lysis Tubes, 2 x 96 Preps/Unit
Product Overview
- Unbiased lysis of microbes including gram +/- bacteria, fungi, protozoans, & viruses from any sample including feces, soil, water, swabs, etc
- Ultra-Pure: High quality DNA/RNA (including small/micro RNAs) are inhibitor-free and ready for qPCR and microbiome measurements using NGS
- High-Sensitivity: Increased detection limit of very low abundance organisms
Technical Specifications
| Brand | Zymo Research Corporation |
| Binding Capacity | 15µg total RNA per 30µl MagBinding Beads. |
| Equipment Required | Magnetic stand, heat block, liquid handler or robotic sample processor (user provided). |
| Purity | High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc. |
| Storage | RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage. |
| Sample Preservation | DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures. |
| Sample Types | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from </= 50mg of mammalian feces, </= 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs. |
| Size Limits | Total RNA including small/microRNAs >/= 17nt. |
Detailed Description
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Citations
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