CAT#:
11-617C
Zymo Research
ZymoBIOMICS™ 96 MagBead DNA Kits
No Lysis Matrix, 2 x 96 Preps/Unit
Zymo Research, 4 x 96 Preps/Unit
Zymo Research, 96 Preps/Unit
Lysis Rack, 2 x 96 Preps/Unit
Lysis Tubes, 2 x 96 Preps/Unit
Product Overview
- High-throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluid
- Lysis system enables efficient and unbiased lysis of microbes including Gram-positive/negative bacteria, fungi, protozoans, algae, etc.
- The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 preps
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | As little as 37.5 µl DNA elution buffer or DNase free water |
| Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 well magnetic stand, liquid handler or other robotic sample processor, 96 well plate heat block. |
| Processing Volume | Fecal up to 100 mg, Soil up to 200 mg, Cells up to 20 mg (Approximately equal to 2x10^8 bacterial, 2x10^7 yeast, or 2x10^6 mammalian cells) |
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage | Eluted DNA should be stored at at least -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Type | Total DNA |
| Yield | Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) |
Detailed Description
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Citations
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