CAT#:
11-321MD
Zymo Research
Quick-DNA Fungal/Bacterial Kits
Zymo Research Kit, 50 Preps/Unit
Zymo Research, 25 Preps/Unit
Zymo Research, 2 x 96 Preps/Unit
Zymo Research, 50 Preps/Unit
Product Overview
- Simple, efficient isolation of DNA from all types of tough-to-lyse fungi and bacteria in minutes.
- Ultra-high density BashingBeads™ are fracture resistant and chemically inert.
- DNA Recovery: Typically, up to ~125 µg total DNA is eluted into as little as 150 µl DNA Elution Buffer per sample.
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 150 μl |
| Equipment | Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter |
| Processing Time | ≤ 20 minutes |
| Processing Volume | ≤ 500mg fungi or bacteria (wet weight), 5x109 bacterial cells, 5x108 yeast cells, or 5x107 mammalian cells |
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 |
| Sample Source | Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| Size Range | Capable of recovering genomic DNA up to and above ≥40 kb. Typical fragment sizes range from 25 to 35 kb. If present, parasitic and viral DNA will also be recovered. |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Type | Total DNA |
| Yield | ≤ 125 μg total DNA |
Technical Documents
Detailed Description
The Quick-DNA Fungal/Bacterial kits are designed for the simple and rapid isolation of DNA from tough-to-lyse fungi as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
Citations
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