CAT#:
11-321
Zymo Research
Quick-DNA Fungal/Bacterial Kits
Zymo Research Kit, 50 Preps/Unit
Zymo Research, 25 Preps/Unit
Zymo Research, 2 x 96 Preps/Unit
Zymo Research, 50 Preps/Unit
Product Overview
- Simple, efficient isolation of DNA (up to 25 µg/prep) from all types of tough-to-lyse fungi (e.g., yeast) and bacteria in as little as 15 minutes
- State-of-the-art, ultra-high density BashingBeads™ are fracture resistant and chemically inert
- Can be used with any bead mill, disrupter, or vortex that can accommodate standard 2 ml tubes
- Omits the use of organic denaturants as well as proteinases
- Processing Time: 10 min
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 35 μl |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer |
| Processing Time | ≤ 15 minutes |
| Processing Volume | ≤100 mg fungi or bacteria (wet weight), 109 bacterial cells, 108 yeast cells, or 107 mammalian cells |
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 |
| Sample Source | Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| Size Range | Capable of recovering genomic DNA sized fragments from up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Type | Total DNA |
| Yield | ≤ 25 μg total DNA |
Detailed Description
The Quick-DNA Fungal/Bacterial kits are designed for the simple and rapid isolation of DNA from tough-to-lyse fungi as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
Citations
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