CAT#:
13-020
Intact Genomics
HB101 Chemically Competent Cells
Chemically Competent Cells, 24 x 100µl/Unit
Chemically Competent Cells, 6 x 100µl/Unit
Product Overview
- Suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning
- Contains the recA13 mutation that minimizes recombination and helps insert stability
- Carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes
- Includes: ig™ JM109(DE3) competent cells, pUC19 control DNA, and recovery medium
Technical Specifications
| Competent Cell Type | Chemically competent |
| Derivative Of | HB101 |
| Species | E. coli |
| Format | Tubes |
| Transformation Efficiency | ?1 x 10^8 cfu/µg pUC19 DNA |
| Blue/white Screening | No |
| Shipping Condition | Dry ice |
Technical Documents
Detailed Description
Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening.
Citations
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