CAT#:
11-334SK
Zymo Research
EZ DNA Methylation Kits
MagPrep, 8 x 96 Rxns/Unit
MagPrep, 4 x 96 Rxns/Unit
Zymo Research Kit, 50 Rxns/Unit
Spin Column Format, 50 Preps/Unit
Shallow-Well, 2 x 96 Rxns/Unit
Zymo Research Kit, 2 x 96 Rxns/Unit
Zymo Research, 200 Rxns/Unit
Product Overview
- A complete system for DNA methylation detection!
- DNA bisulfite treatment, robust hot-start PCR, and a universally methylated human control DNA standard with primers
- Designed for the first time user requiring a consolidated product to perform DNA methylation analysis
- Conversion Efficiency: > 99.5%
- Processing Time: 4 hrs
- DNA Recovery: > 80%
- Spin column format
Technical Specifications
| Applicable For | Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc. |
| Conversion | > 99.5% |
| Elution Volume | ≥ 10 μl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | Samples containing between 50 pg to 2 μg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells. |
| Processing Time | 4 hours |
| Recovery | > 80% |
| Sample Source | Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. |
Technical Documents
Detailed Description
The EZ DNA Methylation Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The EZ DNA Methylation Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Innovative desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for Next-generation sequencing, PCR amplification, endonuclease digestion, sequencing, microarrays, etc. The EZ DNA Methylation Kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Citations
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