CAT#:
11-334M
Zymo Research
EZ DNA Methylation Kits
MagPrep, 8 x 96 Rxns/Unit
MagPrep, 4 x 96 Rxns/Unit
Zymo Research Kit, 50 Rxns/Unit
Spin Column Format, 50 Preps/Unit
Shallow-Well, 2 x 96 Rxns/Unit
Zymo Research Kit, 2 x 96 Rxns/Unit
Zymo Research, 200 Rxns/Unit
Product Overview
- Proven procedure for complete bisulfite conversion of DNA.
- High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Technical Specifications
| Conversion | > 99% |
| Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. |
| Input | 500 pg - 2 μg of DNA |
| Processing Time | 12-16 hours |
| Recovery | > 70% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Detailed Description
The EZ DNA Methylation Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The EZ DNA Methylation Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Innovative desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for Next-generation sequencing, PCR amplification, endonuclease digestion, sequencing, microarrays, etc. The EZ DNA Methylation Kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Citations
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