CAT#:
11-336D
Zymo Research
EZ DNA Methylation-Gold Kits
Deep-Well, 2 x 96 Rxns/Unit
MagPrep, 4 x 96 Rxns/Unit
Zymo Research, 50 Rxns/Unit
MagPrep, 8 x 96 Rxns/Unit
Zymo Research, 200 Rxns/Unit
Shallow-Well, 2 x 96 Rxns/Unit
Product Overview
- High-throughput (96-well) bisulfite conversion of GC-rich DNA in less than 3 hours.
- A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
- Desulfonation and recovery of bisulfite-treated DNA with a 96-well plate.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Technical Specifications
| Conversion | >99% |
| Elution Volume | ≥ 15 μl for Deep-well ≥ 30 μl for Shallow-well |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers |
| Input | 500 pg - 2 μg of DNA. |
| Processing Time | 3 hours |
| Recovery | >75% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Detailed Description
The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation to complement chemical denaturation in the previous protocol. Also, this kit have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. .
Citations
Powered by Bioz