CAT#:
11-335B
Zymo Research
EZ DNA Methylation-Gold Kits
Deep-Well, 2 x 96 Rxns/Unit
MagPrep, 4 x 96 Rxns/Unit
Zymo Research, 50 Rxns/Unit
MagPrep, 8 x 96 Rxns/Unit
Zymo Research, 200 Rxns/Unit
Shallow-Well, 2 x 96 Rxns/Unit
6 product variations: View Options
Product Overview
- High-throughput (96-well) bisulfite conversion of GC-rich DNA in less than 3 hours.
- A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
- Desulfonation and recovery of bisulfite-treated DNA with a 96-well plate.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Product Overview
- Complete, high-throughput, bisulfite conversion of GC-rich DNA in less than 3 hours.
- A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
- High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA
- Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
Product Overview
- Complete bisulfite conversion of GC-rich DNA in less than 3 hours
- A coupled heat denaturation/conversion reaction step steamlines the conversion of non-methylated cytosines into uracil
- Desulfonation and recovery of bisulfite-treated DNA with a spin column or 96-well plate
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc
- Binding Capacity 5 µg/prep
- Processing Time: 3 hrs
- Conversion Efficiency: >99%
- DNA Recovery: >75%
Product Overview
- Complete, high-throughput, bisulfite conversion of GC-rich DNA in less than 3 hours.
- A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
- High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA
- Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
Product Overview
- Complete bisulfite conversion of GC-rich DNA in less than 3 hours
- A coupled heat denaturation/conversion reaction step steamlines the conversion of non-methylated cytosines into uracil
- Desulfonation and recovery of bisulfite-treated DNA with a spin column or 96-well plate
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc
- Binding Capacity 5 µg/prep
- Processing Time: 3 hrs
- Conversion Efficiency: >99%
- DNA Recovery: >75%
Product Overview
- High-throughput (96-well) bisulfite conversion of GC-rich DNA in less than 3 hours.
- A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
- Desulfonation and recovery of bisulfite-treated DNA with a 96-well plate.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Technical Specifications
| Conversion | >99% |
| Elution Volume | ≥ 15 μl for Deep-well ≥ 30 μl for Shallow-well |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers |
| Input | 500 pg - 2 μg of DNA. |
| Processing Time | 3 hours |
| Recovery | >75% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99% |
| Elution Volume | ≥ 25 μl |
| Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. |
| Input | 500 pg - 2 μg of DNA. |
| Processing Time | 3 hours |
| Recovery | > 70% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | >99% |
| Elution Volume | ≥ 10 μl |
| Equipment | Microcentrifuge and thermocycler with heated lid |
| Input | 500 pg - 2 μg of DNA. |
| Processing Time | 3 hours |
| Recovery | >75% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99% |
| Elution Volume | ≥ 25 μl |
| Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. |
| Input | 500 pg - 2 μg of DNA. |
| Processing Time | 3 hours |
| Recovery | > 70% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | >99% |
| Elution Volume | ≥ 10 μl |
| Equipment | Microcentrifuge and thermocycler with heated lid |
| Input | 500 pg - 2 μg of DNA. |
| Processing Time | 3 hours |
| Recovery | >75% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | >99% |
| Elution Volume | ≥ 15 μl for Deep-well ≥ 30 μl for Shallow-well |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers |
| Input | 500 pg - 2 μg of DNA. |
| Processing Time | 3 hours |
| Recovery | >75% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Documents
Technical Documents
Technical Documents
Technical Documents
Technical Documents
Detailed Description
The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation to complement chemical denaturation in the previous protocol. Also, this kit have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. .
6 Product Options
Catalog Number:
11-336D
List Price:
$1035.62
Your Price:
$887.67
Specifications:
| Quantity | 2 x 96 Reactions - Deep Well |
| Description | Deep-Well |
| Qty/Unit | 2 x 96 Rxns/Unit |
Catalog Number:
11-336M
List Price:
$1661.43
Your Price:
$1424.07
Specifications:
| Quantity | 4 x 96 Reactions - MagPrep |
| Description | MagPrep |
| Qty/Unit | 4 x 96 Rxns/Unit |
Catalog Number:
11-335
List Price:
$341.33
Your Price:
$292.57
Specifications:
| Quantity | 50 Reactions |
| Description | Zymo Research |
| Qty/Unit | 50 Rxns/Unit |
Catalog Number:
11-336MB
List Price:
$2604.9
Your Price:
$2232.77
Specifications:
| Quantity | 8 x 96 Reactions - MagPrep |
| Description | MagPrep |
| Qty/Unit | 8 x 96 Rxns/Unit |
Catalog Number:
11-335B
List Price:
$1126.94
Your Price:
$965.96
Specifications:
| Quantity | 200 Reactions |
| Description | Zymo Research |
| Qty/Unit | 200 Rxns/Unit |
Catalog Number:
11-336S
List Price:
$1045.97
Your Price:
$896.54
Specifications:
| Quantity | 2 x 96 Reactions - Shallow Well |
| Description | Shallow-Well |
| Qty/Unit | 2 x 96 Rxns/Unit |
| Catalog # | Description | Qty/Unit | Quantity | Price (USD) | Quantity | Add to Order | |
|---|---|---|---|---|---|---|---|
| 11-335 | Zymo Research | 50 Rxns/Unit | 50 Reactions | ||||
| 11-335B | Zymo Research | 200 Rxns/Unit | 200 Reactions | ||||
| 11-336D | Deep-Well | 2 x 96 Rxns/Unit | 2 x 96 Reactions - Deep Well | ||||
| 11-336M | MagPrep | 4 x 96 Rxns/Unit | 4 x 96 Reactions - MagPrep | ||||
| 11-336MB | MagPrep | 8 x 96 Rxns/Unit | 8 x 96 Reactions - MagPrep | ||||
| 11-336S | Shallow-Well | 2 x 96 Rxns/Unit | 2 x 96 Reactions - Shallow Well |
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