CAT#:
13-042
Intact Genomics
BL21(DE3) Competent Cells
Electrocompetent Cells, 12 x 50µl/Unit
Chemically Competent Cells, 12 x 50µl/Unit
Chemically Competent Cells, 6 x 200µl/Unit
Electrocompetent Cells, 12 x 100µl/Unit
Chemically Competent Cells, 24 x 50µl/Unit
Product Overview
- Suitable for high efficiency transformation and routine protein expression
- Contains >1 x 1010 cfu/µg efficiency with electroporation
- Widely used host background
- Has T7 Expression Strain
- Deficient in both lon (1) and ompT proteases
- Resistant to phage T1 (fhuA2)
- B Strain
- Includes: BL21(DE3) electrocompetent cells, pUC19 control DNA, and recovery medium
Technical Specifications
| Competent Cell Type | Electrocompetent |
| Derivative Of | BL21 (DE3) |
| Species | E. coli |
| Format | Tubes |
| Transformation Efficiency | ?1 x 10^10 cfu/µg pUC19 DNA |
| Blue/white Screening | Yes |
| Shipping Condition | Dry ice |
Detailed Description
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high efficiency transformation protocol listed below. Transformation efficiency should be ? 1×10^9 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
Citations
Powered by Bioz