CAT#:
11-553B
Zymo Research
ZymoPURE™ Plasmid Miniprep Kits
up to 100µg Recovery, 10 Preps/Unit
up to 100µg Recovery, 50 Preps/Unit
up to 100µg Recovery, 800 Preps/Unit
up to 100µg Recovery, 400 Preps/Unit
up to 100µg Recovery, 100 Preps/Unit
5 product variations: View Options
Product Overview
- Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.
- Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl.
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Product Overview
- Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.
- Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl.
- Features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl)
- Optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA.
- Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Product Overview
- Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.
- Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl.
- Features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl)
- Optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA.
- Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Product Overview
- Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.
- Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl.
- Features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl)
- Optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA.
- Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Product Overview
- Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.
- Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl.
- Features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl)
- Optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA.
- Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Technical Specifications
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
| Binding Capacity | 100 μg |
| Culture Input | ≤ 5 mL |
| Elution Volume | 25 μl |
| Endotoxin Levels | ≤ 1 EU/μg |
| Equipment | Microcentrifuge and/or vacuum manifold |
| Format | Spin Column |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
| Recommended Sample Volume | ≤ 5.0 ml |
| Size Range | Up to ~200 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 100 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
Technical Specifications
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
| Binding Capacity | 100 μg |
| Culture Input | ≤ 5 mL |
| Elution Volume | 25 μl |
| Endotoxin Levels | ≤ 1 EU/μg |
| Equipment | Microcentrifuge and/or vacuum manifold |
| Format | Spin Column |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
| Recommended Sample Volume | ≤ 5.0 ml |
| Size Range | Up to ~200 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 100 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
Technical Specifications
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
| Binding Capacity | 100 μg |
| Culture Input | ≤ 5 mL |
| Elution Volume | 25 μl |
| Endotoxin Levels | ≤ 1 EU/μg |
| Equipment | Microcentrifuge and/or vacuum manifold |
| Format | Spin Column |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
| Recommended Sample Volume | ≤ 5.0 ml |
| Size Range | Up to ~200 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 100 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
Technical Specifications
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
| Binding Capacity | 100 μg |
| Culture Input | ≤ 5 mL |
| Elution Volume | 25 μl |
| Endotoxin Levels | ≤ 1 EU/μg |
| Equipment | Microcentrifuge and/or vacuum manifold |
| Format | Spin Column |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
| Recommended Sample Volume | ≤ 5.0 ml |
| Size Range | Up to ~200 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 100 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
Technical Specifications
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
| Binding Capacity | 100 μg |
| Culture Input | ≤ 5 mL |
| Elution Volume | 25 μl |
| Endotoxin Levels | ≤ 1 EU/μg |
| Equipment | Microcentrifuge and/or vacuum manifold |
| Format | Spin Column |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
| Recommended Sample Volume | ≤ 5.0 ml |
| Size Range | Up to ~200 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 100 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
Technical Documents
Technical Documents
Technical Documents
Technical Documents
Detailed Description
Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
5 Product Options
Catalog Number:
11-556
List Price:
$65.27
Your Price:
$55.94
Specifications:
| Quantity | 10 Preps |
| Description | up to 100µg Recovery |
| Qty/Unit | 10 Preps/Unit |
Catalog Number:
11-553S
List Price:
$194.92
Your Price:
$167.08
Specifications:
| Quantity | 50 Preps |
| Description | up to 100µg Recovery |
| Qty/Unit | 50 Preps/Unit |
Catalog Number:
11-553C
List Price:
$2290.11
Your Price:
$1962.95
Specifications:
| Quantity | 800 Preps |
| Description | up to 100µg Recovery |
| Qty/Unit | 800 Preps/Unit |
Catalog Number:
11-553B
List Price:
$1310.43
Your Price:
$1123.23
Specifications:
| Quantity | 400 Preps |
| Description | up to 100µg Recovery |
| Qty/Unit | 400 Preps/Unit |
Catalog Number:
11-553
List Price:
$362.06
Your Price:
$310.34
Specifications:
| Quantity | 100 Preps |
| Description | up to 100µg Recovery |
| Qty/Unit | 100 Preps/Unit |
| Catalog # | Description | Qty/Unit | Quantity | Price (USD) | Quantity | Add to Order | |
|---|---|---|---|---|---|---|---|
| 11-553 | up to 100µg Recovery | 100 Preps/Unit | 100 Preps | ||||
| 11-553B | up to 100µg Recovery | 400 Preps/Unit | 400 Preps | ||||
| 11-553C | up to 100µg Recovery | 800 Preps/Unit | 800 Preps | ||||
| 11-553S | up to 100µg Recovery | 50 Preps/Unit | 50 Preps | ||||
| 11-556 | up to 100µg Recovery | 10 Preps/Unit | 10 Preps |
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