Product Overview
- The fastest spin-column based procedure for purifying up to 10mg of ultra-pure endotoxin-free transfection-grade plasmid DNA
- Innovative ZymoPURE binding technology enables elution of the highest concentration of plasmid DNA directly from a spin-column using a centrifuge
- Routinely recover 1 µg/µl or more plasmid DNA that is ideal for transfection and other sensitive downstream applications
- Eluted DNA is ultrapure and well suited for transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, etc
- Colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization
- Innovative EndoZero™ technology enables transfection in sensitive cells and in vivo research (
- Recovery Volume: as little as 2 ml of ZymoPURE Elution Buffer or DNase free water
- Plasmid DNA Size: Up to 25 kb, Processing Time: 50 min
Technical Specifications
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
| Binding Capacity | 25 mg |
| Culture Input | ≤ 2.5 L |
| Elution Volume | ≥ 3.0 ml |
| Endotoxin Levels | ≤ 0.025 EU/μg of Plasmid DNA |
| Equipment | Vacuum/vacuum manifold and swinging bucket centrifuge |
| Format | Spin-Column |
| Processing Time | ≤ 45 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230 ≥ 2.0 |
| Recommended Sample Volume | ≤ 2.5 L |
| Size Range | Up to 200 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 3.0 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 150 ml of overnight culture grown in LB for high copy number plasmids are 600 – 1,200 μg. |
Detailed Description
The ZymoPURE™ Plasmid Gigaprep kit features a spin column-based method for the purification of up to 10 mg of high-quality plasmid DNA in less than 50 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 2.5 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
As an added convenience, the ZymoPURE™ Plasmid Gigaprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
1 Product Options
Catalog Number:
11-560
List Price:
$1081.92
Your Price:
$927.36
Specifications:
| Quantity | 2 x 96 Preps (No Lysis Matrix) |
| Description | up to 10mg Recovery |
| Qty/Unit | 5 Preps/Unit |
!