









CAT#:
11-742
Zymo Research
ZymoPURE™ 96 Plasmid Miniprep Kits
2 x 96 prep, 192 Preps/Unit
4 x 96 prep, 384 Preps/Unit
2 product variations: View Options
Product Overview
- High-Throughput: Perform 96 Plasmid Minipreps Simultaneously using a Vacuum Manifold or Centrifuge
- Highest Yield: Up to 100 µg of Highly Concentrated Plasmid DNA
- Ultra-Pure: Eluted Plasmid DNA is Transfection-Grade and Free of Cross-Contamination
- Endotoxin Levels: 1 EU/µg or less
- Processing Time: 60 minutes or less
- Size Range:Up to ~200 kb
- Recommended Sample Volume: 5.0 mL or less
Product Overview
- High-Throughput: Perform 96 Plasmid Minipreps Simultaneously using a Vacuum Manifold or Centrifuge
- Highest Yield: Up to 100 µg of Highly Concentrated Plasmid DNA
- Ultra-Pure: Eluted Plasmid DNA is Transfection-Grade and Free of Cross-Contamination
- Endotoxin Levels: 1 EU/µg or less
- Processing Time: 60 minutes or less
- Size Range:Up to ~200 kb
- Recommended Sample Volume: 5.0 mL or less
Technical Specifications
Binding Capacity | 100 µg |
Culture Input | 5 mL |
Elution Volume | 125 µL |
Endotoxin Levels | 1 EU/µg |
Format | 96-Well Plate |
Processing Time | 60 min |
Technical Specifications
Binding Capacity | 100 µg |
Culture Input | 5 mL |
Elution Volume | 125 µL |
Endotoxin Levels | 1 EU/µg |
Format | 96-Well Plate |
Processing Time | 60 min |
Detailed Description
The ZymoPURE™ 96 Plasmid Miniprep Kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. The unique vacuum plate design provides zero buffer retention and low elution volumes. In addition, the provided wash plate also significantly reduces plasmid DNA cross-contamination between wells relative to other popular high-throughput plasmid prep kits. ZymoPURE™ technology uses a modified alkaline lysis method and our patented binding chemistry, which enables the highest DNA binding capacity and rapid loading of the lysate and wash buffer, resulting in the purification of highly concentrated (up to 1 µg/µl) plasmid DNA directly from a 96-well vacuum plate. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR and other sensitive downstream applications.
As an added convenience, the ZymoPURE™ 96 Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. ZymoPURE™ Filter Plates are also included for rapid clearing of the lysate and the unique Zymo-Spin P-96 Plate design allows the binding step to be performed using a vacuum or centrifuge.
Citations
2 Product Options
Catalog Number:
11-741
List Price:
$750.48
Your Price:
$643.27
Specifications:
Quantity | 2 x 96 Preps |
Description | 2 x 96 prep |
Qty/Unit | 192 Preps/Unit |
Catalog Number:
11-742
List Price:
$1326.13
Your Price:
$1136.69
Specifications:
Quantity | 4 x 96 Preps |
Description | 4 x 96 prep |
Qty/Unit | 384 Preps/Unit |
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