CAT#:
11-371
Zymo Research
ZymoBIOMICS™ Spike-in Control (High Microbial Load)
Zymo Research, 250 Preps/Unit
Zymo Research, 25 Preps/Unit
Product Overview
- Absolute Quantification: enables absolute cell number measurements using Next-Generation Sequencing
- in situ Quality Control: ensures each sample is quantified accurately
- Unique composition: comprised of two microbes alien to the human microbiome
Technical Specifications
| Biosafety | This product is not biohazardous as microbes have been fully inactivated. |
| Impurity Level | 0.01% foreign microbial DNA |
| Reference Genomes | Complete reference genomes and 16S rRNA genes |
| Sample Storage | -80°C |
| Storage Solution | DNA/RNA Shield |
| Supplemental Info | Absolute Cell Quantification |
Detailed Description
ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can expose potential bias during DNA extraction. Moreover, with accurately quantified cell numbers, this standard enables absolute cell number quantification in microbiome measurements.
Citations
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