Product Overview
- Rapid, robust, and simple purification of high quality, inhibitor-free total RNA (including small/micro RNAs)
- Samples include feces, soil, water, biofilms, swabs, saliva, and body fluids, etc.
- ZymoBIOMICS™ innovative lysis system enables efficient and unbiased lysis of microbes including gram positive/negative bacteria
- DNA-free RNA is ready for use in any downstream application. DNase I included.
Technical Specifications
| Equipment | Microcentrifuge, vortex, cell disrupter (recommended) |
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water. |
| Size Range | Total RNA ≥17 nt |
| Supplemental Info | Sample Preservation: The Origin of Bias |
| Yield | 100 μg RNA (binding capacity), ≥50 μl (elution volume) |
Detailed Description
The ZymoBIOMICS™ RNA Mini Kit is designed for purifying RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. gram negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield™ preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin® column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids) and is ready for RT-PCR, hybridization, sequencing, etc.
1 Product Options
Catalog Number:
11-600R
List Price:
$908.68
Your Price:
$778.87
Specifications:
| Quantity | 50 Preps |
| Description | Capped Columns |
| Qty/Unit | 50 Preps/Unit |
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