ZymoBIOMICS™ DNA Miniprep Kits
No Lysis Matrix, 50 Preps/Unit
ZymoBIOMICS™ DNA Kit<br>No Lysis Matrix<br>ZymoBIOMICS 96 DNA Kit (No Lysis Matrix), 2 x 96 Preps/Unit
Zymo Research, 2 x 96 Preps/Unit
- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
- Simple Workflow: Simply bead-beat sample, purify via spin-plate, and filter to remove PCR inhibitors. No precipitation or lengthy incubations!
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 20 μl DNA Elution Buffer or DNase free water |
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer |
| Processing Volume | Fecal ≤100 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Supplemental Info | How to Use Microbial Community Standards Tips & Tricks to Reduce Bioburden Score More Spore DNA |
| Type | Total DNA |
| Yield | Up to 5 μg total DNA can be eluted into 20 μl |
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
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