Zymo-Seq cfDNA WGBS Library Prep Kits
Zymo-Seq cfDNA WGBS Library Prep Kits
CAT#: 11-618B Zymo Research

Zymo-Seq cfDNA WGBS Library Prep Kits

Zymo Research, 96 Preps/Unit

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$7,181.80
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Product Overview
  • Optimized for small fragment input
  • Direct ligation-based protocol allows for accurate methylation calling
  • Prepare robust methyl-seq libraries in as little as 3 steps
Technical Specifications
Bisulfite Conversion?99.5% of non-methyllated cytosine residues are converted to uracil; ?99.5% protecton of methylated cytosines.
Equipment RequiredThermal cycler(s) with temperature adjustable lids, microcentrifuge, and magnetic stand
Hands-On Time?2 hours
Total Processing Time?6 hours
Input QualityFor optimal results, use at least minimum input of purified cfDNA with no RNA or gDNA contamination. cfDNA can be concentrated using the DNA Clean & Concentrator (D4013) prior to processing. Input of cfDNA can be suspended in water, DNA Elution Buffer, or TE buffer.
Library StorageLibraries eluted in DNA Elution Buffer (provided) may be stored at ?4°C overnight or ?-20°C for long-term storage.
Minimum Input5 ng
Maximum Input10 ng
Sample Input MaterialPurified cell-free DNA (cfDNA)
Sequencing CompatibilityLibraries are compatible with all Illumina sequencing platforms. Recommended: NextSeq, NovaSeq.
Detailed Description
Zymo-Seq Cell Free DNA WGBS Library Kit provides an optimized and reliable workflow for whole genome bisulfite sequencing (WGBS) library preparation from cell-free DNA (cfDNA). This all-inclusive kit features a straightforward procedure capable of preparing high-quality methyl-seq libraries from as little as 5 ng of cfDNA. The process is completed in three basic steps: bisulfite conversion, direct adapter ligation, and index PCR amplification. The initial bisulfite treatment is gentle on cfDNA yet effective in converting any unmodified cytosines into uracil. Next, the innovative splinted adapters capture and directly ligate the Illumina-compatible adapters onto any size DNA fragment, allowing for sequencing of nicked, damaged, and short DNA fragments that may have otherwise been discarded with traditional library prep methods. The direct ligation of the adapters also eliminates the need for second strand synthesis, end repair, and dA tailing steps, thus reducing bias by preserving the integrity of any native methylation that is present on the fragment termini. Finally, the adapter-ligated cfDNA is indexed and amplified via PCR with Zymo-Seq UDIs, generating libraries that are ready for sequencing on any Illumina instrument.

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Catalog Number: 11-618B
List Price: $8378.77
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Quantity 96 Preps
Description Zymo Research
Qty/Unit 96 Preps/Unit
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11-618 Zymo Research 24 Preps/Unit 24 Preps
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11-618B Zymo Research 96 Preps/Unit 96 Preps
List Price: $8,378.77
Online Price: $7,181.80

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