CAT#:
11-384H
Zymo Research
ZR DNA Sequencing Clean-up Kits
Zymo Research, 2 x 96 Preps/Unit
Zymo Research, 200 Preps/Unit
Zymo Research, 4 x 96 Preps/Unit
Zymo Research, 50 Preps/Unit
Product Overview
- Complete elimination of """"Dye Blobs"""" for high quality Phred scores and long read lengths
- Flexible 15-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps
- Reusable 96-Well Filtration Plates that Support Both Gel and Capillary Electrophoresis Platforms
- Simple 10 Minute Bind, Wash, Elute Procedure
Technical Specifications
| Elution Volume | ≥ 15 μl of DNA Elution Buffer |
| Equipment | Centrifuge with microplate carriers |
| Sample Source | Sequencing input samples with post-cycle sequencing reaction contaminants from DNA extension products |
| Sample Storage | Eluted DNA can be used immediately or stored at ≤ -20°C |
| Yield | Up to 5 μg DNA |
Detailed Description
The ZR DNA Sequencing Clean-Up Kits provide a simple and rapid (2 & 10 minute) method for removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data, including dye peaks or "dye blobs" which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide.
Citations
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