CAT#:
11-326
Zymo Research
RNA Clean & Concentrator-5 Kit
Zymo Research Kit, 200 Preps/Unit
Zymo Research Kit, 50 Preps/Unit
Product Overview
- Quick 5 min. method for cleaning and concentrating RNA from any enzymatic reaction
- Yields up to 5 µg RNA in as little as 6 µl elution volume with No wash residue carryover
- Ideal for purification of RNA from aqueous phase after acid phenol extraction
- Isolation of broad range total RNA (>17 nt to >200 nt). Alternate tunable procedures
Technical Specifications
| Equipment | Microcentrifuge |
| Format | Spin-Column |
| Purity | RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
| Sample Source | DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction |
| Size Range | Total RNA ≥ 17 nt |
| Supplemental Info | Tips & Tricks For RNA Isolation How to Design Primers for PCR Experiments What is RNA-Seq? |
| Yield | 10 μg RNA (binding capacity), ≥ 6 μl (elution volume) |
Detailed Description
The RNA Clean & Concentrator-5 kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality, RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
Citations
Powered by Bioz