Quick-DNA Miniprep Plus Kits
Zymo Research, 50 Preps/Unit
Zymo Research, 2 x 96 Preps/Unit
Zymo Research, 200 Preps/Unit
Zymo Research, 10 Preps/Unit
- Extract high-quality DNA easily and reliably from any sample source
- Samples: biological fluids, cultured/monolayer cells, solid tissues, etc.
- DNA can be eluted into as little as 35ul DNA Elution Buffer or water.
- The DNA binding capacity of the column is 25ug
- Overview: Utilize a Proteinase K Digestion and Clean-Spin™ Column for effective recovery of DNA.
- Capable of recovering genomic and mitochondrial DNA sized fragments >50kb
- DNA Purity: ready for all sensitive applications such as PCR, genotyping, Next-Gen seq, etc.
- Input: up to 200ull biological fluid, 5 x 10^6 cells, 25mg solid tissue
| Elution Volume | ≥ 35 μl |
| Equipment | Water bath or heat block (55°C), microcentrifuge, and vortex. |
| Purity | High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0). |
| Size Range | Capable of recovering genomic and mitochondrial DNA sized fragments > 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered. |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Workflow | Utilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA. |
| Yield | The DNA binding capacity of the column is 25 μg. Typically, mammalian tissues yield: 1-3 μg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 μg DNA per mg liver and kidney. Human whole blood will yield 3-7 μg DNA per 100 μl blood sampled. |
The Quick-DNA Miniprep Plus Kit is the easiest DNA extraction kit for high yield ultra-pure total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. The Quick-DNA Miniprep Plus DNA extraction kit comes equipped with innovative reagents and Zymo-Spin Technology, allowing for ultra-pure DNA (> 50 kb) to be eluted in as little as 35 µl. Zymo-Spin Columns ensure that there is zero buffer retention, enabling ultra-pure DNA recovery in a simple 20-minute protocol. Purified DNA is RNA-free, removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. The extracted DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
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