CAT#:
11-317C
Zymo Research
Quick-DNA Miniprep Kits
Capped Columns, 50 Preps/Unit
Capped columns, 200 Preps/Unit
2 product variations: View Options
Product Overview
- Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs or cultured cells
- Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
- Unique extraction technology excludes the use of Proteinase K and organic denaturants.
- Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
- DNA Recovery: Up to 25 µg total DNA is eluted into as little as 30 µl DNA Elution Buffer or water.
- Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled.
- Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues.
- 3-5 µg DNA per mg liver, kidney and lung tissues.
Product Overview
- Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs or cultured cells
- Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
- Unique extraction technology excludes the use of Proteinase K and organic denaturants.
- Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
- DNA Recovery: Up to 25 µg total DNA is eluted into as little as 30 µl DNA Elution Buffer or water.
- Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled.
- Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues.
- 3-5 µg DNA per mg liver, kidney and lung tissues.
Technical Specifications
| Elution Volume | ≥50 μl |
| Equipment | Microcentrifuge, Vortex |
| Purity | High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 |
| Sample Source | Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized. |
| Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Workflow | Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. |
| Yield | Up to 25 μg total DNA is eluted into ≥50 μl (30 μl minimum) DNA Elution Buffer or water. Human whole blood yields 3-7 μg DNA per 100 μl blood sampled. Mammalian tissues already homogenized will yield 1-3 μg DNA per mg. |
Technical Specifications
| Elution Volume | ≥50 μl |
| Equipment | Microcentrifuge, Vortex |
| Purity | High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 |
| Sample Source | Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized. |
| Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Workflow | Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. |
| Yield | Up to 25 μg total DNA is eluted into ≥50 μl (30 μl minimum) DNA Elution Buffer or water. Human whole blood yields 3-7 μg DNA per 100 μl blood sampled. Mammalian tissues already homogenized will yield 1-3 μg DNA per mg. |
Technical Documents
Detailed Description
The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
2 Product Options
Catalog Number:
11-317AC
List Price:
$221.31
Your Price:
$189.69
Specifications:
| Quantity | 50 Preps |
| Description | Capped Columns |
| Qty/Unit | 50 Preps/Unit |
Catalog Number:
11-317C
List Price:
$714.17
Your Price:
$612.15
Specifications:
| Quantity | 200 Preps |
| Description | Capped columns |
| Qty/Unit | 200 Preps/Unit |
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