Product Overview
- Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues.
- Zymo-Spin™ Technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
Technical Specifications
| Elution Volume | ≤ 10 μl |
| Equipment | Water bath or heat block (55°C), microcentrifuge, and vortex. |
| Purity | High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0). |
| Size Range | Capable of recovering genomic and mitochondrial DNA sized fragments > 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered. |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Workflow | Utilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA. |
| Yield | The DNA binding capacity of the well is 5 μg. Typically, mammalian tissues yield: 1-3 μg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 μg DNA per mg liver and kidney. Human whole blood will yield 3-7 μg DNA per 100 μl blood sampled. |
Technical Documents
Detailed Description
The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral), cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA > 50 kb to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Citations
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