CAT#:
11-322M
Zymo Research
Quick-DNA Fecal/Soil Kits
Zymo Research, 2 x 96 Preps/Unit
Zymo Research, 50 Preps/Unit
Zymo Research, 25 Preps/Unit
Zymo Research Kit, 50 Preps/Unit
4 product variations: View Options
Product Overview
- Rapid methods for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes including those from humans, birds, rats, mice, cattl
- Ultra-high density BashingBeads™ are fracture resistant and chemically inert.
- Fast-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
- Sample Sources: Bacterial, protist, as well as host DNA can be effectively isolated from a ?80 mg sample of mammalian feces.
- Capable of recovering genomic DNA sized fragments from 100 bp to ?40 kb.
- Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
Product Overview
- Rapid methods for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes including those from humans, birds, rats, mice, etc
- Ultra-high density BashingBeads™ are fracture resistant and chemically inert
- Fast-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product
- Processing Time: 15 min
- DNA Size Limits: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be
- Sample Sources: Bacterial, protist, as well as host DNA can be effectively isolated from 150 mg or less of sample of mammalian feces.
- DNA Recovery: Up to 5 ug total DNA is eluted into as little as 10 ul DNA Elution Buffer per sample.
Product Overview
- Rapid methods for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes including those from humans, birds, rats, mice, etc
- Ultra-high density BashingBeads™ are fracture resistant and chemically inert
- Fast-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product
- Processing Time: 25 min
- DNA Size Limits: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be
- Sample Sources: Bacterial, protist, as well as host DNA can be effectively isolated from 375 mg or less of sample of mammalian feces.
- DNA Recovery: Up to 125 ?g total DNA is eluted into as little as 150 ?l DNA Elution Buffer per sample.
Product Overview
- Rapid methods for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes including those from humans, birds, rats, mice, etc
- Ultra-high density BashingBeads™ are fracture resistant and chemically inert
- Fast-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product
- Processing Time: 15 min
- DNA Size Limits: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be
- Sample Sources: Bacterial, protist, as well as host DNA can be effectively isolated from 150 mg or less of sample of mammalian feces.
- DNA Recovery: Up to 25 ug total DNA is eluted into as little as 25 ul DNA Elution Buffer per sample.
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 50 μl |
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer. |
| Processing Volume | ≤80mg feces, ≤ 135 mg soil, ≤20 mg fungal/bacterial cells (wet weight) |
| Purity | A260/A280 nm ≥1.8. |
| Sample Source | Feces or soil |
| Sample Storage | DNA stored at ≤ -20°C. |
| Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Type | Total DNA |
| Yield | ≤ 5 μg total DNA |
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 20 μl |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer |
| Processing Volume | ≤50 mg feces, ≤ 250 mg soil, ≤20 mg fungal/bacterial cells (wet weight) |
| Purity | A260/A280 nm ≥1.8. |
| Sample Source | Feces or soil |
| Sample Storage | DNA stored at ≤ -20°C. |
| Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Type | Total DNA |
| Yield | ≤ 5 μg total DNA |
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 150 μl |
| Equipment | Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter |
| Processing Volume | ≤375 mg feces, ≤ 5 g soil, ≤ 500 mg fungal/bacterial cells (wet weight) |
| Purity | A260/A280 nm ≥1.8. |
| Sample Source | Feces or soil |
| Sample Storage | DNA stored at ≤ -20°C. |
| Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Type | Total DNA |
| Yield | ≤ 125 μg total DNA |
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 50 μl |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer |
| Processing Volume | ≤150 mg feces, ≤ 250 mg soil, ≤100 mg fungal/bacterial cells (wet weight) |
| Purity | A260/A280 nm ≥1.8. |
| Sample Source | Feces or soil |
| Sample Storage | DNA stored at ≤ -20°C. |
| Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Type | Total DNA |
| Yield | ≤ 25 μg total DNA |
Technical Documents
Detailed Description
The Quick-DNA Fecal/Soil Microbe Kits are fecal and soil DNA extraction kits designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources including humans, birds, rats, mice, cattle, etc. The procedure of these fecal and soil DNA extraction kits is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin and Zymo MagBinding Bead technologies are then used to isolate the DNA in select fecal and soil DNA extraction kits in this collection. These fecal and soil DNA extraction kits feature our Inhibitor Removal technology, which removes humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
4 Product Options
Catalog Number:
11-322H
List Price:
$1739.9
Your Price:
$1491.35
Specifications:
| Quantity | 2 x 96 Preps |
| Description | Zymo Research |
| Qty/Unit | 2 x 96 Preps/Unit |
Catalog Number:
11-322M
List Price:
$566.36
Your Price:
$485.46
Specifications:
| Quantity | 50 Micro Preps |
| Description | Zymo Research |
| Qty/Unit | 50 Preps/Unit |
Catalog Number:
11-322MD
List Price:
$1233.11
Your Price:
$1056.95
Specifications:
| Quantity | 25 Midi Preps |
| Description | Zymo Research |
| Qty/Unit | 25 Preps/Unit |
Catalog Number:
11-322
List Price:
$483.6
Your Price:
$414.52
Specifications:
| Quantity | 50 Mini Preps |
| Description | Zymo Research Kit |
| Qty/Unit | 50 Preps/Unit |
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