Product Overview
- High-quality cell free RNA is easily and robustly purified from up to 1 ml of plasma, serum, or other biological fluids
- Immediately ready for downstream applications including RT-qPCR and Next-Generation Sequencing
- Sample Sources: Up to 1 ml of plasma, serum, cerebrospinal fluids, or amniotic fluids.
- RNA Recovery: Yields are sample dependent and may vary. Typical RNA recovery ranges from 1 – 100 ng/ml of human plasma or serum.
Technical Specifications
| Elution Volume | cfRNA can be eluted into 15 μl (to maximize total yield) or as little as 6 μl (for highly concentrated eluate) of DNase/RNase-Free Water. |
| Processing Time | Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours. |
| Purity | High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing. |
| Required Equipment | Vacuum manifold, microcentrifuge, vortex, water bath or heating block, and vacuum. |
| Sample Type | Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids. |
| Size | RNA ≥ 17 nt. |
| Yield | Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA. |
Detailed Description
The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.
1 Product Options
Catalog Number:
11-364
List Price:
$967.35
Your Price:
$829.2
Specifications:
| Quantity | 50 Preps |
| Description | Zymo Research |
| Qty/Unit | 50 Preps/Unit |
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