CAT#:
11-616B
Zymo Research
Quick-16S Plus NGS Library Prep Kits
w/ Primer Set 2, 96 Rxns/Unit
w/ Primer Set 3, 96 Rxns/Unit
w/ Primer Set 1, 96 Rxns/Unit
w/ Primer Set 4, 96 Rxns/Unit
Product Overview
- 8x less hands-on time than conventional 16S library prep protocols
- Premixed plate minimizes hands-on time and handling errors
- 100% automation ready with only a single PCR step and no need for normalization
- Only 30 minutes of hands-on time for 96 samples
Technical Specifications
| Amplicon Size | The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ?388 bp. |
| Equipment Required | Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible recommended), or standard PCR system, and 96-well real-time PCR plates. |
| Index Sequences | 10 bp indexes are listed in the MiSeq Sample Sheet Template provided. |
| Sample Input Sequencing Platform | Purified microbial DNA (?100 ng), free of PCR inhibitors. Compatible with all Illumina sequencing platforms using custom sequencing primers. We recommend the MiSeq Reagent Kit v2 (300-cycle). |
| V4 Primer Sequences (adapters Not Included) | 515f (GTGYCAGCMGCCGCGGTAA) and 806r (GGACTACNVGGGTWTCTAAT). |
| V4 Premix Plate | Each position of the premix plate contains Equalase qPCR Premix, water, and uniquely indexed (UDI) 515f and 806r V4 primers mixed at their proper ratios for a 10 µl final reaction volume. |
Detailed Description
The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure® bead-based clean-ups, simplifies the library prep process. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.
Citations
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