CAT#:
17-511C
PCR Biosystems
qPCRBIO Probe Mix Hi-ROX
20 x 1ml Master Mix, 2000 x 20ul Rxns/Unit
1 x 1ml Master Mix, 100 x 20ul Rxns/Unit
5 x 1ml Master Mix, 500 x 20ul Rxns/Unit
50 x 1ml Master Mix, 5000 x 20ul Rxns/Unit
Product Overview
- High efficiency in multiplex reactions
- Extremely fast extension rate produces earliest Ct values
- Universally compatible on all real-time PCR cyclers in both standard and fast cycling conditions
- Amplification from complex templates including GC-rich sequences and low copy number targets
- Can be used in all probe-based real-time PCR assays
- Also available as a blue mix (Cat #: 17-515) with non-reactive blue dye for easy visualization during pipetting
Technical Specifications
| Reactions | 2000 x 20ul Rxns/Unit |
| Packaging Configuration | 20 x 1ml Master Mix |
| Storage | -30 to -15°C for 12 months or 5°C for 1 month |
| Color | Blue |
| Rox Level | Hi-Rox: 1.12nM of 50uM Rox |
| MgCl2 Concentration | Mix contains 12mM for final reaction concentration of 3mM |
| Compare To | TaqMan™ Master Mixes, Qiagen QuantiTect and QuantiNova Probe PCR Kits, NEB Luna® Universal Probe qPCR Master Mix, Promega GoTaq® Probe qPCR Master Mix |
Technical Documents
Detailed Description
Our universal probe qPCR Hi-ROX kit is designed to give superior sensitivity and specificity in all probe-based real-time PCR assays, including TaqMan®, Scorpions® and molecular beacon probes.
Reliably perform singleplex or multiplex expression studies and diagnostic assays with this robust, market-leading probe kit. The optimised mastermix is also suitable for qPCR-based genotyping and allelic discrimination studies.
Citations
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