qMAX Probe One Step 4X RT-qPCR Kit

qMAX Probe One Step 4X RT-qPCR Mix is a high-concentration, one-step reverse transcription quantitative PCR system optimized for sensitive detection of viral RNA and DNA targets. The system comprises a 4× qMAX™ Probe Master Mix and a separate 20× qMAX™ Reverse Transcriptase supplemented with an RNase inhibitor, enabling modular assay design and improved reagent utilization. The 4× master mix format permits increased template input per reaction, enhancing analytical sensitivity for low-copy targets. Additionally, the elevated concentration supports reduced total reaction volumes, facilitating rapid thermal cycling while maintaining amplification efficiency and reproducibility.

The formulation is specifically optimized for dual-labeled hydrolysis probe-based assays and is compatible with both standard and fast cycling protocols. It supports robust performance in Singleplex and multiplex RT-qPCR applications, including higher-order multiplexing (>3 targets). Buffer composition and enzyme system are engineered to maintain amplification efficiency across a broad range of template complexities, including GC-rich and AT-rich regions, while exhibiting tolerance to common PCR inhibitors. To maximize compatibility with multiplex assay designs and diverse instrumentation, the master mix is supplied without ROX passive reference dye, allowing user-defined normalization strategies where required.