CAT#:
11-380H
Zymo Research
Oligo Clean and Concentrator
Zymo Research, 4 x 96 Preps/Unit
Zymo Research, 2 x 96 Preps/Unit
Zymo Research, 50 Preps/Unit
Zymo Research, 200 Preps/Unit
Product Overview
- Quick, high-throughput (96-well) recovery of ultra-pure DNA and RNA oligonucleotides.
- Complete removal of dyes, salts, enzymes, nucleotides, and short oligos.
- As little as 10 µl elution with zero retention Fast Spin plates.
- Eluted DNA/RNA is well suited for use in hybridization, sequencing, PCR, ligation, etc.
Technical Specifications
| Applicable For | Single-stranded and double-stranded DNA and RNA |
| Elution Volume | ≥ 10 μl |
| Equipment | Centrifuge with microplate carriers |
| Purity | A260/A280 > 1.8, A260/A230 > 1.8 |
| Sample Source | Enzymatic reaction mixtures containing oligonucleotides ≥ 16 nt (radioactive-, biotin-, DIG-labeled, etc.) |
| Size Range | For oligonucleotides ≥ 16 nt, up to 23 kb |
| Yield | 10 μg of ssDNA/RNA or 5 μg of dsDNA with a typical recovery > 90% |
Detailed Description
The Oligo Clean & Concentrator provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥ 16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure. DNA/RNA is washed and concentrated into a small volume of water. Purified DNA/RNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
Citations
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