Nickel Metal Affinity Resin Cross-linked Beads, 6%
Cross-linked Beads, 6%, 25ml/Unit
Cross-linked Beads, 6%, 100ml/Unit
- Ready-to-use IMAC resin that utilizes nickel (Ni2+) for purifying 6x His-tagged proteins
- Works well with relatively easy to separate His-tagged proteins
- Allows for purification of proteins under native or denaturing conditions
- High capacity: >50mg protein per ml resin
- Ligand density: 20-40umol Ni2+/ml resin
- Bead specifications: Spherical, ~50 - 150um
- Available in bulk or in prepacked 1ml or 5ml gravity flow columns
- For batch or column purifications
- Ready-to-use IMAC resin that utilizes nickel (Ni2+) for purifying 6x His-tagged proteins
- Works well with relatively easy to separate His-tagged proteins
- Allows for purification of proteins under native or denaturing conditions
- High capacity: >50mg protein per ml resin
- Ligand density: 20-40umol Ni2+/ml resin
- Bead specifications: Spherical, ~50 - 150um
- Available in bulk or in prepacked 1ml or 5ml gravity flow columns
- For batch or column purifications
Prometheus™ Ni-IDA Agarose resin is an affinity chromatography resin for purification of histidine-tagged proteins by Immobilized Metal Affinity Chromatography (IMAC). The resin utilizes nickel (Ni2+) for the purification of 6x histidine tagged proteins and binds to six histidine residues (6x His), a common tag used in protein purification. It consists of iminodiacetate (IDA - a tridentate chelating agent) coupled to 6% cross-linked agarose beads. The IDA binds divalent nickel ion with a capacity of 20-40umoles Ni2+/ml resin. The protein binding capacity is >50mg protein per ml resin, but we have demonstrated binding of 117mg of purified DHAK-(6XHis) tagged protein to a ml of resin.
2 Product Options
Specifications:
| Size | 25 ml |
| Description | Cross-linked Beads, 6% |
| Qty/Unit | 25ml/Unit |
Specifications:
| Size | 100 ml |
| Description | Cross-linked Beads, 6% |
| Qty/Unit | 100ml/Unit |
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