Ni-NTA Agarose Beads Cross-linked Beads, 6%
Cross-linked Beads, 6%, 100ml/Unit
Cross-linked Beads, 6%, 500ml/Unit
Cross-linked Beads, 6%, 25ml/Unit
- High performance nickel-IMAC resin for one step purification of His-tagged proteins
- Four metal-binding sites on the chelate enable high-protein binding and low-metal ion leaching
- Allows for purification of proteins under native or denaturing conditions
- Recover His-tagged proteins expressed in baculovirus, yeast, mammalian and bacterial cells
- High capacity: >50mg protein per ml resin
- Bead specifications: Spherical, ~50 - 150um
- Most common IMAC resin for working in reducing conditions
- Offers more specific binding than Ni-IDA Agarose
Prometheus™ Ni-NTA Agarose resin is is a high-capacity, high-performance nickel-IMAC resin specifically designed for affinity purification of His-tagged (6XHis) fusion proteins. It can be used under native and denaturing conditions to recover His-tagged proteins from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells. Prometheus™ Ni-NTA Agarose uses nitrilotriacetic acid (NTA - a tetradenate chelating ligand) in a cross-linked 6% agarose matrix and is loaded with divalent nickel ions (Ni2+). The resin provides exceptional binding capacity of >50mg/ml of resin and performance that equals or exceeds that of Ni-NTA resins from other suppliers.
Ni-NTA resins are the most common choice for 6xHis-tag protein purifications by IMAC because of the four metal-binding sites on the chelate, which enable high-protein binding and low-metal ion leaching. The purity and yield of the target protein varies and is dependent on many factors including working conditions, size, conformation, expression levels, solubility and sample impurities.
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