EZ RNA Methylation Kits
Zymo Research Kit, 200 Preps/Unit
Zymo Research Kit, 50 Preps/Unit
- Fast and reliable bisulfite conversion of RNA for methylation analysis
- Specifically optimized for complete conversion of non-methylated cytosine in RNA
- Ideal for all RNA inputs
- Conversion efficiency: > 99% of non-methylated C residues are converted to U with > 99% protection of 5-methylcytosine
- RNA recovery: > 80%
- Fast and reliable bisulfite conversion of RNA for methylation analysis
- Specifically optimized for complete conversion of non-methylated cytosine in RNA
- Ideal for all RNA inputs
- Conversion efficiency: > 99% of non-methylated C residues are converted to U with > 99% protection of 5-methylcytosine
- RNA recovery: > 80%
| Conversion | > 99% |
| Elution Volume | ≥ 10 μl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | 0.5 - 1 μg of RNA. |
| Processing Time | 50 Minutes |
| Purity | Purified, converted RNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. |
| Recovery | > 80% |
| Conversion | > 99% |
| Elution Volume | ≥ 10 μl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | 0.5 - 1 μg of RNA. |
| Processing Time | 50 Minutes |
| Purity | Purified, converted RNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. |
| Recovery | > 80% |
The EZ RNA Methylation™ Kit features rapid and reliable bisulfite treatment and conversion of cytosines in RNA for methylation analysis. The kit streamlines the three-step process for complete conversion of cytosine in into uracil. RNA denaturation and bisulfite conversion processes are combined into a single step. No buffer preparation is necessary. The RNA Conversion Reagent is provided ready-to-use: simply add the reagent to an RNA sample and incubate as indicated.Also, innovative in-column desulphonation technology eliminates messy precipitation steps, ensuring researchers obtain consistent results. The product has been designed to minimize template degradation, loss of RNA during treatment and clean-up, and to provide complete conversion of cytosine for accurate methylation analysis. Recovered RNA is ideal for RT-PCR, sequencing, library preparation and Next-Gen sequencing.
2 Product Options
Specifications:
| Quantity | 200 Preps |
| Description | Zymo Research Kit |
| Qty/Unit | 200 Preps/Unit |
Specifications:
| Quantity | 50 Preps |
| Description | Zymo Research Kit |
| Qty/Unit | 50 Preps/Unit |
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