CAT#:
11-337D
Zymo Research
EZ DNA Methlyation-Direct Kits
MagPrep, 8 x 96 Rxns/Unit
MagPrep, 4 x 96 Rxns/Unit
Deep-Well, 2 x 96 Rxns/Unit
Zymo Research Kit, 50 Rxns/Unit
Zymo Research, 200 Rxns/Unit
Shallow-Well, 2 x 96 Rxns/Unit
Product Overview
- Complete bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples.
- Compatible with small sample inputs as few as 10 cells or 50 pg DNA.
- Desulfonation and recovery of bisulfite-treated DNA with a spin column.
Technical Specifications
| Conversion | > 99.5% |
| Elution Volume | Shallow well: > 30 μl Deep-well: >15 μl |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers. |
| Input | Samples containing between 50 pg to 2 μg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells. |
| Processing Time | 4 hours |
| Recovery | > 80% |
| Sample Source | Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Detailed Description
The EZ DNA Methylation-Direct kit features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Citations
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