2 product variations: View Options
Product Overview
- Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
- Convenient 1 hour, single-enzyme digest vs. conventional 6-16 hours multi-step enzyme digestion protocols
Product Overview
- Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
- Convenient 1 hour, single-enzyme digest vs. conventional 6-16 hours multi-step enzyme digestion protocols
Technical Specifications
| Assay Condition | DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour |
| Concentration | 5 U/μl |
| Enzyme Inactivation | Heat inactivate enzyme at 70C for 20 minutes |
| Storage | Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C. |
| Unit Definition | 5 U of enzyme will digest 1 μg of genomic DNA in 25 μl reaction volume at 37C for ≥ 1 hr |
Technical Specifications
| Assay Condition | DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour |
| Concentration | 5 U/μl |
| Enzyme Inactivation | Heat inactivate enzyme at 70C for 20 minutes |
| Storage | Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C. |
| Unit Definition | 5 U of enzyme will digest 1 μg of genomic DNA in 25 μl reaction volume at 37C for ≥ 1 hr |
Technical Documents
Detailed Description
DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
2 Product Options
Catalog Number:
11-266
List Price:
$938.05
Your Price:
$804.05
Specifications:
| Units | 1000 Units |
| Description | Zymo Research |
| Qty/Unit | 1000 U/Unit |
Catalog Number:
11-265
List Price:
$293.45
Your Price:
$251.54
Specifications:
| Units | 250 Units |
| Description | Zymo Research |
| Qty/Unit | 250 U/Unit |
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