CAT#:
11-302
Zymo Research
DNA Clean & Concentrator-5µg Kits
PCR Purification Kit, 5µg/Well, 2 x 96 Preps/Unit
PCR Purification Kit (5µg), 200 Preps/Unit
Zymo Research, 10 Preps/Unit
PCR Purification Kit (5µg), 50 Preps/Unit
PCR Purification Kit, 5µg/Well, 4 x 96 Preps/Unit
PCR Purification Kit (5µg), 50 Preps/Unit
PCR Purification Kit (5µg), 200 Preps/Unit
Product Overview
- Clean and concentrate (e.g., PCR purification) up to 5 µg DNA in as little as 6µl elution in 2 minutes with 0µl wash residue carryover
- Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc
- DNA Size Limits: 50 bp to 23 kb
- DNA Recovery: As much as 5ug. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.
- Sample Sources: DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
Technical Specifications
| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| Elution Volume | ≥ 6 μl |
| Equipment | Microcentrifuge |
| Purity | A260/A280 > 1.8 |
| Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
| Size Range | 50 bp to 23 kb |
| Yield | ≤ 5 μg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
Detailed Description
The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
Citations
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