CAT#:
11-304C
Zymo Research
DNA Clean & Concentrator-25µg Kits
PCR Purification Kit (25µg), 50 Preps/Unit
PCR Purification Kit (25µg), 200 Preps/Unit
PCR Purification Kit (25µg), 200 Preps/Unit
PCR Purification Kit (25µg), 50 Preps/Unit
Product Overview
- Clean and concentrate (e.g., PCR purification) up to 25 µg DNA in as little as 25µl elution in 2 minutes with 0µl wash residue carryover
- Column design allows DNA to be eluted at high concentrations into minimal volumes as little as 25ul
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc
- DNA Size Limits: 50 bp to 23 kb
- DNA Recovery: As much as 25ug. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.
- Sample Sources: DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
Technical Specifications
| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| Elution Volume | ≥ 25 μl |
| Equipment | Microcentrifuge |
| Purity | Highly-pure DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
| Size Range | 50 bp to 23 kb |
| Yield | ≤ 25 μg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
Detailed Description
The DNA Clean & Concentrator-25 (DCC-25) is a PCR purification kit designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin column. This PCR purification kit features Zymo-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
Citations
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