CAT#:
84-207
Cytiva
Cytiva® HisTrap™ HP
Prepacked Column, 1 x 1 mL/Unit
Prepacked Column, 1 x 5 mL/Unit
Prepacked Columns, 5 x 5 mL/Unit
Prepacked Columns, 5 x 1 mL/Unit
4 product variations: View Options
Product Overview
- High binding capacity, at least 40 mg/ml chromatography medium
- High resolution: due to small bead size (average bead size is 34 µm)
- Negligible nickel leakage: helps retain activity and minimize protein precipitation
- Broad reagent compatibility: suitable for use with a wide range of reducing agents, detergents, denaturants, and other additives
- Convenient HiTrap format: 1 mL and 5 mL columns compatible with syringe, pump, or chromatography systems such as ÄKTA or other FPLC systems
Product Overview
- High binding capacity, at least 40 mg/ml chromatography medium
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Negligible Ni2+ leakage
- Simple manual operation with a syringe, pump, or chromatography system such as ÄKTA design
Product Overview
- High binding capacity, at least 40 mg/ml chromatography medium
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Negligible Ni2+ leakage
- Simple manual operation with a syringe, pump, or chromatography system such as ÄKTA design
Product Overview
- High binding capacity, at least 40 mg/ml chromatography medium
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Negligible Ni2+ leakage
- Simple manual operation with a syringe, pump, or chromatography system such as ÄKTA design
Technical Documents
Technical Documents
Technical Documents
Detailed Description
His-tag protein purification by IMAC is both popular and highly efficient. HisTrap HP columns are packed with Ni Sepharose High Performance (HP) affinity resin. This resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. The chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups. HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)1. The shorter (histidine)4 will bind more weakly and the longer (histidine)1 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)1 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be copurified with the tagged target protein.
4 Product Options
Catalog Number:
84-207
List Price:
$90.8
Your Price:
$77.9
Specifications:
| Pack Size | 1 x 1 mL |
| Description | Prepacked Column |
| Qty/Unit | 1 x 1ml/Unit |
Catalog Number:
84-208
List Price:
$290.3
Your Price:
$228.45
Specifications:
| Pack Size | 1 x 5 mL |
| Description | Prepacked Column |
| Qty/Unit | 1 x 5ml/Unit |
Catalog Number:
84-210
List Price:
$1163.25
Your Price:
$892.5
Specifications:
| Pack Size | 5 x 5 mL |
| Description | Prepacked Columns |
| Qty/Unit | 5 x 5ml/Unit |
Catalog Number:
84-209
List Price:
$343.45
Your Price:
$269.9
Specifications:
| Pack Size | 5 x 1 mL |
| Description | Prepacked Columns |
| Qty/Unit | 5 x 1ml/Unit |
!