Cytiva® GSTrap™ Fast Flow
Prepacked Column, 1 x 5 mL/Unit
Prepacked Columns, 5 x 1 mL/Unit
Prepacked Columns, 5 x 5 mL/Unit
Prepacked Columns, 2 x 1 mL/Unit
- Fast and simple one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- Easy scaleup by connecting the columns in series
- Sepharose 4 Fast Flow provides good flow properties
- Fast and simple one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- Easy scaleup by connecting the columns in series
- Sepharose 4 Fast Flow provides good flow properties
- Fast and simple one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- Easy scaleup by connecting the columns in series
- Sepharose 4 Fast Flow provides good flow properties
- Fast and simple one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- Easy scaleup by connecting the columns in series
- Sepharose 4 Fast Flow provides good flow properties
GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
The medium, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.
4 Product Options
Specifications:
| Pack Size | 1 x 5 mL |
| Description | Prepacked Column |
| Qty/Unit | 1 x 5ml/Unit |
Specifications:
| Pack Size | 5 x 1 mL |
| Description | Prepacked Columns |
| Qty/Unit | 5 x 1ml/Unit |
Specifications:
| Pack Size | 5 x 5 mL |
| Description | Prepacked Columns |
| Qty/Unit | 5 x 5ml/Unit |
Specifications:
| Pack Size | 2 x 1 mL |
| Description | Prepacked Columns |
| Qty/Unit | 2 x 1ml/Unit |