CAT#:
11-379H
Zymo Research
ChIP DNA Clean & Concentrator Kits
Zymo Research, 4 x 96 Preps/Unit
Uncapped Columns, 50 Preps/Unit
Capped Columns, 50 Preps/Unit
Zymo Research, 2 x 96 Preps/Unit
Product Overview
- Rapid high throughput (96-well) recovery of ultra-pure DNA from chromatin immunoprecipitation (ChIP), cell lysates, Proteinase K digested samples, PCR
- Plate design permits DNA elution at high concentrations into minimal volumes as little as 10 µl/well.
- Omits the use of organic solvents and the need for ethanol precipitation.
- Eluted DNA is well suited for use in PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications.
Technical Specifications
| Elution Volume | ≥ 10 μl |
| Processing Time | 15 minutes |
| Recovery | 70-90% for DNA 50 bp - 10 kb 70% for DNA > 10 kb |
| Size Range | 50 bp - 23 kb |
| Storage | All components should be stored at room temperature. |
Technical Documents
Detailed Description
The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator provides a hassle-free method for the rapid purification and concentration of high-quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes. The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP.
Citations
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