CAT#:
42-138B
Apex Bioresearch Products
Apex Taq RED Master Mix, 2X, 1.5mM MgCl2 (Final Conc.)
1.5mM MgCl2 (Final Conc.), 500 Reactions/Unit
1.5mM MgCl2 (Final Conc.), 1000 Reactions/Unit
1.5mM MgCl2 (Final Conc.), 100 Reactions/Unit
Product Overview
- Apex 2X RED Taq Master Mix, 1.5mM MgCl2 [Final Conc.] with ammonium buffer that promotes robust amplification, high yield and high specificity
- Optimized mix for specific PCR results - Just add template, primers and water for significantly reduced set-up time and reduced risk of contamination
- Contains Apex state-of-the-art thermostable recombinant DNA Polymerase that reliably processes up to 5kb with high product yield
- Exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang convenient for direct T-A cloning
- Inert red dye and stabilizer allow easy pipetting and direct loading onto your agarose gel for analysis
- Dye front runs at 300 - 1000 bp on 0.5 - 1.5 % agarose gel
- Multiple 1.25ml aliquot packaging prevents repetitive freeze-thaw cycles - mix is also stable refrigerated (4ºC) for at least 6 months without risk
- Total volume: 25.0ml (1000 x 50µl Reactions)
Technical Specifications
| Product Overhang | 3'-dA |
| Hot-Start | No |
| 5' - 3' Exonuclease Activity | Yes |
| Concentration | 2X |
| Loading Dye | Yes |
| Storage | Long term storage (3 years) at -20°C Short term storage (6 months) at 4°C |
Technical Documents
Detailed Description
Apex Taq Red Master Mix is a ready-to-use 2.0x reaction mix. It contains Apex Taq DNA Polymerase, an Ammonium based Buffer System, Apex ultra pure dNTPs (>99%) and Magnesium Chloride, and a Red Dye for easy visualization. Simply add primers, template, and water to successfully carry out primer extension and other molecular biology applications. The number of reactions is based on using 25ul of the master mix for a final reaction volume of 50ul. If desired, the reaction size may be scaled down to a final volume of 20 or 25ul by using 10ul or 12.5ul of the master mix respectively.
Citations
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