CAT#:
42-149
Apex Bioresearch Products
Apex Hot Start Taq BLUE Master Mix, 2X, 1.5mM MgCl2 (Final) w/ Buffer II
1.5mM MgCl2 (Final), Buffer II, 2500 Reactions/Unit
1.5mM MgCl2 (Final), Buffer II, 100 Reactions/Unit
1.5mM MgCl2 (Final), Buffer II, 500 Reactions/Unit
1.5mM MgCl2 (Final), Buffer II, 1000 Reactions/Unit
4 product variations: View Options
Product Overview
- Apex Hot Start Master Mix, Blue, 1.5mM MgCl2 (Final Conc.) with balanced potassium/ammonium buffer system II
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- Same as clear version, but with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Dye front runs at 100-500 bp (0.5 -1.5 % agarose gel). PCR products can be used for sequencing
- Multiple 1.25ml aliquot packaging prevent repetitive freeze-thaw cycles - mix is stable refrigerated (4ºC) for at least 6 months without risk
Product Overview
- Apex Hot Start Master Mix, Blue, 1.5mM MgCl2 (Final Conc.) with balanced potassium/ammonium buffer system II
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- Same as clear version, but with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Dye front runs at 100-500 bp (0.5 -1.5 % agarose gel). PCR products can be used for sequencing after PCR Clean-up
- Multiple 1.25ml aliquot packaging prevent repetitive freeze-thaw cycles - mix is stable refrigerated (4ºC) for at least 6 months without risk
Product Overview
- Apex Hot Start Master Mix, Blue, 1.5mM MgCl2 (Final Conc.) with balanced potassium/ammonium buffer system
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- Same as clear version, but with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Dye front runs at 100-500 bp (0.5 -1.5 % agarose gel). PCR products can be used for sequencing after PCR Clean-up
- Multiple 1.25ml aliquot packaging prevent repetitive freeze-thaw cycles - mix is stable refrigerated (4ºC) for at least 6 months without risk
Product Overview
- Apex Hot Start Master Mix, Blue, 1.5mM MgCl2 (Final Conc.) with balanced potassium/ammonium buffer system
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- Same as clear version, but with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Dye front runs at 100-500 bp (0.5 -1.5 % agarose gel). PCR products can be used for sequencing after PCR Clean-up
- Multiple 1.25ml aliquot packaging prevent repetitive freeze-thaw cycles - mix is stable refrigerated (4ºC) for at least 6 months without risk
Technical Documents
Technical Documents
Technical Documents
Technical Documents
Detailed Description
Apex Hot Start Master Mix BLUE is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. Apex Hot Start DNA Polymerase, balanced KCL/NH4 buffer system, dNTPs and magnesium chloride are present in Apex Hot Start Master Mix w/ Buffer II for multiplex PCR. Each reaction requires 25uL of the 2.0X reaction mix.
Apex Hot Start DNA Polymerase is a modified form of Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature.
4 Product Options
Catalog Number:
42-150
List Price:
$3880.33
Your Price:
$3408.46
Specifications:
| Size | 2500 Reactions |
| Description | 1.5mM MgCl2, Buffer II |
| Qty/Unit | 2500 Reactions/Unit |
Catalog Number:
42-147
List Price:
$206.06
Your Price:
$180.06
Specifications:
| Size | 100 Reactions |
| Description | 1.5mM MgCl2, Buffer II |
| Qty/Unit | 100 Reactions/Unit |
Catalog Number:
42-148
List Price:
$812.42
Your Price:
$713.46
Specifications:
| Size | 500 Reactions |
| Description | 1.5mM MgCl2, Buffer II |
| Qty/Unit | 500 Reactions/Unit |
Catalog Number:
42-149
List Price:
$1595.54
Your Price:
$1391.77
Specifications:
| Size | 1000 Reactions |
| Description | 1.5mM MgCl2, Buffer II |
| Qty/Unit | 1000 Reactions/Unit |
| Catalog # | Description | Qty/Unit | Size | Price (USD) | Quantity | Add to Order | |
|---|---|---|---|---|---|---|---|
| 42-147 | 1.5mM MgCl2, Buffer II | 100 Reactions/Unit | 100 Reactions | ||||
| 42-148 | 1.5mM MgCl2, Buffer II | 500 Reactions/Unit | 500 Reactions | ||||
| 42-149 | 1.5mM MgCl2, Buffer II | 1000 Reactions/Unit | 1000 Reactions | ||||
| 42-150 | 1.5mM MgCl2, Buffer II | 2500 Reactions/Unit | 2500 Reactions |
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