CAT#:
42-197
Apex Bioresearch Products
Apex Hot Start Master Mix, 2X, 1.5mM MgCl2 (Final)
1.5mM MgCl2 (Final), 100 Reactions/Unit
1.5mM MgCl2 (Final), 6000 Reactions/Unit
1.5mM MgCl2 (Final), 500 Reactions/Unit
1.5mM MgCl2 (Final), 1000 Reactions/Unit
4 product variations: View Options
Product Overview
- Apex Hot Start Master Mix, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, high yield and high specificity
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- BLUE version also available (42-145) with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Multiple 1.25ml aliquot packaging prevents repetitive freeze-thaw cycles, Total Volume: 2.5ml
- Mix is stable refrigerated (4ºC) for at least 6 months without risk
Product Overview
- Apex Hot Start Master Mix, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, high yield and high specificity
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- BLUE version also available (42-145) with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Mix is stable refrigerated (4ºC) for at least 6 months without risk
Product Overview
- Apex Hot Start Master Mix, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, high yield and high specificity
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- BLUE version also available (42-145) with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Multiple 1.25ml aliquot packaging prevents repetitive freeze-thaw cycles, Total Volume: 12.5ml
- Mix is stable refrigerated (4ºC) for at least 6 months without risk
Product Overview
- Apex Hot Start Master Mix, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, high yield and high specificity
- Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
- Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
- screening, GC-rich, multiplex and direct colony PCR
- Reliably processes up to 5kb with high product yield, exhibits 5' to 3 exonuclease activity and leaves a 3'dA overhang
- BLUE version also available (42-145) with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
- Multiple 1.25ml aliquot packaging prevents repetitive freeze-thaw cycles, Total Volume: 25ml
- Mix is stable refrigerated (4ºC) for at least 6 months without risk
Technical Specifications
| Product Overhang | 3'-dA |
| Hot-Start | Yes |
| 5' - 3' Exonuclease Activity | Yes |
| Concentration | 2X |
| Loading Dye | No |
| Storage | Long term storage (3 years) at -20°C Short term storage (6 months) at 4°C |
Technical Specifications
| Product Overhang | 3'-dA |
| Hot-Start | Yes |
| 5' - 3' Exonuclease Activity | Yes |
| Concentration | 2X |
| Loading Dye | No |
| Storage | Long term storage (3 years) at -20°C Short term storage (6 months) at 4°C |
Technical Specifications
| Product Overhang | 3'-dA |
| Hot-Start | Yes |
| 5' - 3' Exonuclease Activity | Yes |
| Concentration | 2X |
| Loading Dye | No |
| Storage | Long term storage (3 years) at -20°C Short term storage (6 months) at 4°C |
Technical Documents
Technical Documents
Technical Documents
Detailed Description
Apex Hot Start Master Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. Apex Hot Start DNA Polymerase is a modified form of Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Apex Hot Start DNA Polymerase, ammonium buffer system, dNTPs and magnesium chloride are present in Apex Hot Start Master Mix with Apex Buffer I. Each reaction requires 25ul of the 2.0X reaction mix.
For most standard applications Master Mix based on Ammonium Buffer I is the best option. It promotes robust amplification, high yield and high specificity. In certain cases Master Mix with Buffer II is the best option.
4 Product Options
Catalog Number:
42-197
List Price:
$204.92
Your Price:
$180.06
Specifications:
| Size | 100 Reactions |
| Description | 1.5mM MgCl2, Buffer I (NH4) |
| Qty/Unit | 100 Reactions/Unit |
Catalog Number:
42-198C
List Price:
$8938.28
Your Price:
$7652.65
Specifications:
| Size | 6000 Reactions |
| Description | 1.5mM MgCl2, Buffer I (NH4) |
| Qty/Unit | 3 x 50ml/Unit |
Catalog Number:
42-198
List Price:
$868.4
Your Price:
$763.94
Specifications:
| Size | 500 Reactions |
| Description | 1.5mM MgCl2, Buffer I (NH4) |
| Qty/Unit | 500 Reactions/Unit |
Catalog Number:
42-198B
List Price:
$1461.31
Your Price:
$1266.46
Specifications:
| Size | 1000 Reactions |
| Description | 1.5mM MgCl2, Buffer I (NH4) |
| Qty/Unit | 1000 Reactions/Unit |
| Catalog # | Description | Qty/Unit | Size | Price (USD) | Quantity | Add to Order | |
|---|---|---|---|---|---|---|---|
| 42-197 | 1.5mM MgCl2, Buffer I (NH4) | 100 Reactions/Unit | 100 Reactions | ||||
| 42-198 | 1.5mM MgCl2, Buffer I (NH4) | 500 Reactions/Unit | 500 Reactions | ||||
| 42-198B | 1.5mM MgCl2, Buffer I (NH4) | 1000 Reactions/Unit | 1000 Reactions | ||||
| 42-198C | 1.5mM MgCl2, Buffer I (NH4) | 3 x 50ml/Unit | 6000 Reactions |
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