Fluorescein-labeled proaerolysin (FLAER) is used in flow cytometry to diagnose paroxysmal nocturnal hemoglobinuria (PNH). It is a unique protein that binds tightly and specifically to mammalian glycol-phosphatidylinositol (GPI) anchored proteins on the cell surface. FLAER fails to bind to lymphocytes, monocytes, and granulocytes in a PNH patient due to the patient's white blood cells losing expression of the GIP anchored cell-surface protein. In addition to antibody-mediated PNH cell detection, FLAER detects ALL GPI-anchored proteins expressed on the surface of leukocytes.
iFluor™ fluorophores are optimized for strong fluorescence, high photostability and pH independence on proteins. They are avaialble to span the full UV-visible-IR spectrum. iFluor™488 is a great alternative to Alexa Fluor® 488, Dylight™ 488 and Vio® 515.
