Polyplus-transfection 101000010 Polyplus-transfection 101000010 PULSin® protein Delivery Reagent, With Hepes Buffer (20mM), 400 µl/unit, With Hepes Buffer (20mM), 400 µl/unit Primary Image
Polyplus-transfection 101000010 Polyplus-transfection 101000010 PULSin® protein Delivery Reagent, With Hepes Buffer (20mM), 400 µl/unit, With Hepes Buffer (20mM), 400 µl/unit Primary Image
Polyplus-transfection 101000010 Polyplus-transfection 101000010 PULSin® protein Delivery Reagent, With Hepes Buffer (20mM), 400 µl/unit, With Hepes Buffer (20mM), 400 µl/unit Secondary Image
title: Examples of proteins, antibodies and peptides delivered to cells using PULSin®.; description:
title: Efficiency of R-phycoerythrin delivery using PULSin® in selected cells.; description:
title: Delivery of Pep-A (Streptococcus TPE B epitope, 16 aa), into HeLa cells.; description: Complexes are formed with Pep-A (1 µg, lissamine-rhodamine derivative, Sigma) and PULSin® (4 µl). Observation is carried out 16 h post-delivery.
title: FACS analysis of HeLa cells after delivery of R-phycoerythrin (upper graph) or FITC-anti-alpha-tubulin antibody (lower graph) with PULSin® or with competitor reagents B and C.; description: Data are presented as histograms of the mean intensity of fluorescence for each cell population.
Polyplus-transfection 101000010 Polyplus-transfection 101000010 PULSin® protein Delivery Reagent, With Hepes Buffer (20mM), 400 µl/unit, With Hepes Buffer (20mM), 400 µl/unit Primary Image
Polyplus-transfection 101000010 Polyplus-transfection 101000010 PULSin® protein Delivery Reagent, With Hepes Buffer (20mM), 400 µl/unit, With Hepes Buffer (20mM), 400 µl/unit Secondary Image
title: Examples of proteins, antibodies and peptides delivered to cells using PULSin®.; description:
title: Efficiency of R-phycoerythrin delivery using PULSin® in selected cells.; description:
title: Delivery of Pep-A (Streptococcus TPE B epitope, 16 aa), into HeLa cells.; description: Complexes are formed with Pep-A (1 µg, lissamine-rhodamine derivative, Sigma) and PULSin® (4 µl). Observation is carried out 16 h post-delivery.
title: FACS analysis of HeLa cells after delivery of R-phycoerythrin (upper graph) or FITC-anti-alpha-tubulin antibody (lower graph) with PULSin® or with competitor reagents B and C.; description: Data are presented as histograms of the mean intensity of fluorescence for each cell population.
CAT#: 55-141 Polyplus-transfection

PULSin® Protein, Antibody, and Peptide Delivery Reagent

With Hepes Buffer (20mM), 400 µl/unit

List Price: $851.00
List Price: $851.00
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 $729.45
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Product Overview
  • Effective and robust delivery into the cytoplasm
  • Delivery to a wide variety of cells including primary cells and Hard-to-transfect cells
  • Highly efficient delivery
  • Easy to use
Technical Specifications
ReagentPULSin®
Molecule DeliveredProtein; Antibody; Peptide
Cell TypesAdherent & suspension cells grown in presence of serum
Number Of Transfection0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates
StorageStore PULSin® at 5 °C ± 3°C. Do not freeze. Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.
Provided WithHepes Buffer (20mM) for protein dilution; R-Phycoerythrin used as a positive control
Technical Documents
Detailed Description
PULSin® protein delivery reagent is dedicated to the delivery of proteins, antibodies and peptides into cells and offers a powerful approach to functional studies. PULSin® can be used to target intracellular proteins with antibodies in living cells without fixation.

Delivery information: Supplied with 20 µg R-Phycoerythrin (positive control) and Hepes dilution buffer. 0,4 ml of PULSin® protein delivery reagent is sufficient for 24 reactions in 6-well plates.

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