MCDB 153 medium is a modification of Ham's nutrient mixture F-12 designed for the growth of non-transformed cell types in a serum-free formulation. Each subcategory contains supplements optimizing a specific cell type. MCDB 153 medium is specific for human epidermal keratinocyte culture, clonal growth of chicken embryo fibroblasts or Chinese Hamster Ovary (CHO) cells using low levels of Fetal Bovine Serum Protein (FBSP), a wide array of trace elements or no serum protein.
