| DNA Purity | High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing. |
| DNA Recovery | Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor. |
| DNA Storage | Eluted DNA should be stored at ≤ -20oC |
| Processing Volume | Plasma – Single centrifugation: up to 3 ml
Plasma – Double centrifugation: up to 10 ml
Serum – up to 10 ml
Amniotic fluid – up to 1 m l
Cerebrospinal fluid (CSF) – up to 1 ml
Saliva – up to 1 ml
Cell-free saliva – up to 5 ml |
| Recovery Volume | ≥ 35 μl of DNA Elution Buffer or DNase free water. |
| Required Equipment | Water bath or heat block (55oC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge. |
| Sample Sources | Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF). |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
