Cat #: 55-311

Polyplus-Transfection 101000033 PEIpro® DNA Transfection Reagent, Large-Scale Virus Production, 10ml/Unit

Polyplus-Transfection 101000033 PEIpro
Polyplus-Transfection 101000033 PEIpro
Polyplus-Transfection 101000033 PEIpro
Polyplus-Transfection 101000033 PEIpro

Cat #: 55-311

Polyplus-Transfection 101000033 PEIpro® DNA Transfection Reagent, Large-Scale Virus Production, 10ml/Unit


Large-Scale Virus Production

10ml/Unit

Brand: Polyplus-transfection

  • Optimized PEI-based transfection reagent for scalable virus production
  • Superior viral titers compared to other commercial PEIs and Calcium Phosphate
  • Suitable for Process Development of large-scale virus production
  • Reliable and secure supply

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Large-Scale Virus Production

10ml/Unit

Brand: Polyplus-transfection

  • Optimized PEI-based transfection reagent for scalable virus production
  • Superior viral titers compared to other commercial PEIs and Calcium Phosphate
  • Suitable for Process Development of large-scale virus production
  • Reliable and secure supply

PEIpro® transfection reagent is the leading chemical-based DNA transfection reagent that offers flexibility and scalability to develop a robust and sustainable Process Development for viral vector production. PEIpro® benefits from extensive research development in PEI polymer chemistry and formulation to achieve highest transfection efficiency in both adherent and suspension cell culture systems. Protocols established with PEIpro® during Process Development are directly implementable for large-scale manufacturing of preclinical and clinical batches of viral vectors with the higher quality grades of PEIpro®: PEIpro®-HQ and PEIpro®-GMP.

With its unique property to efficiently condense several plasmids of different sizes, PEIpro® transfection reagent is ideal for optimal co-transfection of several plasmids containing the gene of interest and necessary viral components to produce full recombinant virions. PEIpro® has benefited from extensive developments that made it the unique PEI-based transfection reagent that can offer the flexibility and robustness that is needed during Process Development and Scale-up; it is compatible with commercially available adherent and suspension virus production systems for lentivirus and AAV viral vectors

Brand Polyplus-Transfection
Reagent Volume 10ml
Sterile Yes
Concentration 1 mg/ml
Molecule Delivered Plasmid DNA and co-delivery of several plasmid DNA
Applications Large-scale virus production
Virus Types Mainly adenovirus, Adeno-Associated Viruses (AAV), Lentivirus, influenza virus, retrovirus, Virus-like particles (VLP)
Cell Types Mammalian producer cell lines (HEK-293, HEK-293 derivatives, BHK, VERO cell lines, virus-specific packaging cell lines.
Number of Transfections 100ml of PEIpro® transfection reagent is sufficient to transfect on average 50 L of cell culture
Storage Condition 5 ± 3°C
Shipping Condition Ambient
Shelf Life 36 months from manufacturing date
Provided with Protocol, certificate of analysis, certificate of origin and non-hazardous product statement

PEIpro® is composed of a linear polyethylenimine (PEI) whose molecular weight is proprietary information. Extensive optimization experiments have been performed to select a linear PEI for optimal DNA delivery with the lowest cytotoxicity in vitro. PEIpro® is provided as an aqueous solution of highly pure and qualified linear PEI at a concentration of 1 mg/L.

PEIpro® is stable for at least two years, when stored appropriately.

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