ZymoPURE™ II Plasmid Maxiprep Kits
Up to 3.0 mgs yield, 10 Preps/Unit
Up to 3.0 mgs yield, 20 Preps/Unit
- Fast: Simple 16-minute protocol
- Highest Yield: Up to 3.0 mg from a spin column
- Ultrapure: Endotoxin-Free and Transfection Ready. For sensitive primary cells, in vivo injections, cloning, etc.
- Simply bind, wash and elute
- Application Ready: (ex. transfection, CRISPR, gene modification, restriction endonuclease digestion, lentiviral vectors, synthetic biology)
- Fast: Simple 16-minute protocol
- Highest Yield: Up to 3.0 mg from a spin column
- Ultrapure: Endotoxin-Free and Transfection Ready. For sensitive primary cells, in vivo injections, cloning, etc.
- Simply bind, wash and elute
- Application Ready: transfection, (ex. CRISPR, gene modification, restriction endonuclease digestion, lentiviral vectors, synthetic biology)
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
| Binding Capacity | 3.0 mg |
| Culture Input | ≤ 150 ml |
| Elution Volume | ≥ 300 μl |
| Endotoxin Levels | ≤ 0.025 EU/μg of Plasmid DNA |
| Equipment | Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge. |
| Format | Spin-Column |
| Processing Time | ≤ 18 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
| Recommended Sample Volume | ≤ 150 ml |
| Size Range | Up to 200 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 3.0 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 150 ml of overnight culture grown in LB for high copy number plasmids are 600 – 1,200 μg. |
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
| Binding Capacity | 3.0 mg |
| Culture Input | ≤ 150 ml |
| Elution Volume | ≥ 300 μl |
| Endotoxin Levels | ≤ 0.025 EU/μg of Plasmid DNA |
| Equipment | Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge. |
| Format | Spin-Column |
| Processing Time | ≤ 18 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
| Recommended Sample Volume | ≤ 150 ml |
| Size Range | Up to 200 kb |
| Supplemental Info | How to Increase Plasmid Yield DOs and DON'Ts of Plasmid Purification |
| Yield | Up to 3.0 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 150 ml of overnight culture grown in LB for high copy number plasmids are 600 – 1,200 μg. |
Product Video
Product Video
The ZymoPURE™ Plasmid Maxiprep kit features a spin column-based method for the purification of up to 3.0 mg of high-quality plasmid DNA with less than 0,025 EU/ug of DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for multiple applications including transfection, CRISPR, gene modification, restriction endonuclease digestion, lentiviral vectors, synthetic biology, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
2 Product Options
Specifications:
| Quantity | 10 Preps |
| Description | up to 1200µg Recovery |
| Qty/Unit | 10 Preps/Unit |
Specifications:
| Quantity | 20 Preps |
| Description | up to 1200µg Recovery |
| Qty/Unit | 20 Preps/Unit |
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