CAT#:
Zymo Research
ZymoBIOMICS™ DNA Microprep Kits
w/Lysis Tubes, 50 Preps/Unit
No Lysis Matrix, 50 Preps/Unit
2 product variations: View Options
Product Overview
- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Product Overview
- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 10 μl ZymoBIOMICS DNase/RNase Free Water |
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). |
| Processing Volume | Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Supplemental Info | How to Use Microbial Community Standards Tips & Tricks to Reduce Bioburden Score More Spore DNA |
| Type | Total DNA |
| Yield | Up to 5 μg total DNA can be eluted into ≥ 10 μl |
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 10 μl ZymoBIOMICS DNase/RNase Free Water |
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). |
| Processing Volume | Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Supplemental Info | How to Use Microbial Community Standards Tips & Tricks to Reduce Bioburden Score More Spore DNA |
| Type | Total DNA |
| Yield | Up to 5 μg total DNA can be eluted into ≥ 10 μl |
Technical Documents
Detailed Description
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads.
This kit is equipped with Zymo's OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
2 Product Options
Catalog Number:
11-600ML
List Price:
$611.8
Your Price:
$524.45
Specifications:
| Quantity | 50 Preps |
| Description | w/Lysis Tubes |
| Qty/Unit | 50 Preps/Unit |
Catalog Number:
11-600M
List Price:
$452.95
Your Price:
$388.3
Specifications:
| Quantity | 50 Preps, No Lysis Matrix |
| Description | No Lysis Matrix |
| Qty/Unit | 50 Preps/Unit |
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