CAT#:
Zymo Research
ZymoBIOMICS™ 96 MagBead DNA Kits
No Lysis Matrix, 2 x 96 Preps/Unit
Zymo Research, 4 x 96 Preps/Unit
Zymo Research, 96 Preps/Unit
Lysis Rack, 2 x 96 Preps/Unit
Lysis Tubes, 2 x 96 Preps/Unit
5 product variations: View Options
Product Overview
- DNA for microbiome or metagenome analyses
- Validated unbiased for microbiome measurements
- Inhibitor-free DNA from any sample
- Certified low bioburden
- No precipitation or lengthy incubations
Product Overview
- Unbiased lysis of microbes including gram +/- bacteria, fungi, protozoans, & viruses from any sample including feces, soil, water, swabs, etc
- Ultra-Pure: High quality DNA/RNA (including small/micro RNAs) are inhibitor-free and ready for qPCR and microbiome measurements using NGS
- High-Sensitivity: Increased detection limit of very low abundance organisms
Product Overview
- Unbiased lysis of microbes including gram +/- bacteria, fungi, protozoans, & viruses from any sample including feces, soil, water, swabs, etc
- Ultra-Pure: High quality DNA/RNA (including small/micro RNAs) are inhibitor-free and ready for qPCR and microbiome measurements using NGS
- High-Sensitivity: Increased detection limit of very low abundance organisms
Product Overview
- DNA for microbiome or metagenome analyses
- Validated unbiased for microbiome measurements
- Inhibitor-free DNA from any sample
- Certified low bioburden
- No precipitation or lengthy incubations
Product Overview
- High-throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluid
- Lysis system enables efficient and unbiased lysis of microbes including Gram-positive/negative bacteria, fungi, protozoans, algae, etc.
- The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 preps
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ? 37.5 µl DNA elution buffer or DNase free water |
| Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 well magnetic stand, liquid handler or other robotic sample processor, 96 well plate heat block. |
| Processing Volume | Fecal ?100 mg, Soil ? 200 mg, Cells ? 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage | Eluted DNA should be stored at ? -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Type | Total DNA |
| Yield | Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) |
Technical Specifications
| Brand | Zymo Research Corporation |
| Binding Capacity | 15µg total RNA per 30µl MagBinding Beads. |
| Equipment Required | Magnetic stand, heat block, liquid handler or robotic sample processor (user provided). |
| Purity | High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc. |
| Storage | RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage. |
| Sample Preservation | DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures. |
| Sample Types | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from </= 50mg of mammalian feces, </= 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs. |
| Size Limits | Total RNA including small/microRNAs >/= 17nt. |
Technical Specifications
| Brand | Zymo Research Corporation |
| Binding Capacity | 15µg total RNA per 30µl MagBinding Beads. |
| Equipment Required | Magnetic stand, heat block, liquid handler or robotic sample processor (user provided). |
| Purity | High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc. |
| Storage | RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage. |
| Sample Preservation | DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures. |
| Sample Types | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from </= 50mg of mammalian feces, </= 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs. |
| Size Limits | Total RNA including small/microRNAs >/= 17nt. |
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ? 37.5 µl DNA elution buffer or DNase free water |
| Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 well magnetic stand, liquid handler or other robotic sample processor, 96 well plate heat block. |
| Processing Volume | Fecal ?100 mg, Soil ? 200 mg, Cells ? 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase free water and is suitable for all downstream applications including PCR and Next-Generation sequencing. |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage | Eluted DNA should be stored at ? -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Type | Total DNA |
| Yield | Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) |
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | As little as 37.5 µl DNA elution buffer or DNase free water |
| Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 well magnetic stand, liquid handler or other robotic sample processor, 96 well plate heat block. |
| Processing Volume | Fecal up to 100 mg, Soil up to 200 mg, Cells up to 20 mg (Approximately equal to 2x10^8 bacterial, 2x10^7 yeast, or 2x10^6 mammalian cells) |
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage | Eluted DNA should be stored at at least -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Type | Total DNA |
| Yield | Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) |
Technical Documents
Technical Documents
Technical Documents
Technical Documents
Detailed Description
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Citations
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5 Product Options
Catalog Number:
11-617B
List Price:
$966.97
Your Price:
$828.83
Specifications:
| Quantity | 2 x 96 Preps (No Lysis Matrix) |
| Description | No Lysis Matrix |
| Qty/Unit | 2 x 96 Preps/Unit |
Catalog Number:
11-724B
List Price:
$2929.19
Your Price:
$2510.73
Specifications:
| Quantity | 4 x 96 Preps |
| Description | Zymo Research |
| Qty/Unit | 4 x 96 preps/Unit |
Catalog Number:
11-724
List Price:
$914.34
Your Price:
$783.72
Specifications:
| Quantity | 1 x 96 Preps |
| Description | Zymo Research |
| Qty/Unit | 96 preps/Unit |
Catalog Number:
11-617A
List Price:
$1430.5
Your Price:
$1226.15
Specifications:
| Quantity | 2 x 96 Preps - w/ Lysis Rack |
| Description | Lysis Rack |
| Qty/Unit | 2 x 96 Preps/Unit |
Catalog Number:
11-617C
List Price:
$1654.66
Your Price:
$1418.28
Specifications:
| Quantity | 2 x 96 Preps (w/ Lysis Tubes) |
| Description | Lysis Tubes |
| Qty/Unit | 2 x 96 Preps/Unit |
| Catalog # | Description | Qty/Unit | Quantity | Price (USD) | Quantity | Add to Order | |
|---|---|---|---|---|---|---|---|
| 11-617A | Lysis Rack | 2 x 96 Preps/Unit | 2 x 96 Preps - w/ Lysis Rack | ||||
| 11-617B | No Lysis Matrix | 2 x 96 Preps/Unit | 2 x 96 Preps (No Lysis Matrix) | ||||
| 11-617C | Lysis Tubes | 2 x 96 Preps/Unit | 2 x 96 Preps (w/ Lysis Tubes) | ||||
| 11-724 | Zymo Research | 96 preps/Unit | 1 x 96 Preps | ||||
| 11-724B | Zymo Research | 4 x 96 preps/Unit | 4 x 96 Preps |
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